Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.

產(chǎn)品簡介SpeciesMouseProtein ConstructionExpressed with an N-terminal Met.PDGF-BB (Ser82-Thr190)Accession # P31240Purity> 95% as analyzed by SDS-PAGEEndotoxin Level< 0.2 EU/μg of protein by gel clotting methodBiological ActivityED50 < 2.5 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 4.0 × 105 U/mg.Expression SystemE. coliApparent Molecular Weight~24.7 kDa, on SDS-PAGE under non-reducing conditions.FormulationLyophilized after extensive dialysis against 10 mM sodium citrate, pH 3.0.ReconstitutionIt is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O up to 100 μg/ml.Storage & StabilityUpon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.